Description
Intended Uses: The 90K/Mac-2 BP ELISA is an enzyme-linked immunosorbent assay for the detection of soluble human 90K/Mac-2 Binding Protein. The 90K/Mac-2 BP ELISA is for research use only.
Principle of the Assay: An anti-90K/Mac-2 BP monoclonal coating antibody is adsorbed onto microwells. 90K/Mac-2 BP present in the sample or standard binds to the antibody adsorbed to the microwells. Following incubation a HRP-conjugated monoclonal anti-90K/Mac-2 BP antibody is added and binds to 90K/Mac-2 BP captured by the first antibody. Unbound enzyme conjugated anti-90K/Mac-2 BP is removed during a wash step and substrate solution reactive with HRP is added to the wells. An anti-90K/Mac-2 BP monoclonal coating antibody is adsorbed onto microwells. 90K/Mac-2 BP present in the sample or standard binds to the antibody adsorbed to the microwells. Following incubation a HRP-conjugated monoclonal anti-90K/Mac-2 BP antibody is added and binds to 90K/Mac-2 BP captured by the first antibody. Unbound enzyme conjugated anti-90K/Mac-2 BP is removed during a wash step and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of soluble 90K/Mac-2 BP present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from five 90K/Mac-2 BP standards and 90K/Mac-2 BP sample concentration determined